Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes. Cellular Localization: Cytoplasmic
Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 0.5-1ug/million cells Immunofluorescence: 1-2ug/ml Immunohistochemistry (Frozen & Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT (No special pretreatment is required for staining of formalin/paraffin tissues.) Optimal dilution for a specific application should be determined. Positive Control: Tonsil or lymph node Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Klonalität:
Monoclonal
Klon-Bezeichnung:
[SPM250]
Isotyp:
IgG1
Reinheit:
Purified by Protein A/G affinity chromatography.
Formulierung:
Supplied as a liquid in 10mM PBS, 0.05% BSA, 0.05% sodium azide. Also available without BSA and azide, see 172103-AF.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
