Cholera Toxin, Vibrio cholerae, Inaba 569B Not for Export

Produktübersicht

• Artikelname: Cholera Toxin, Vibrio cholerae, Inaba 569B Not for Export
• Artikelnummer: USB-215932
• Hersteller Artikelnummer: 215932
• Alternativnummer: USB-215932-1
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Produced by Vibrio cholerae, cholera toxin (CT) is a multimeric enterotoxin that transfers ADP-ribose to a G protein, locking adenyl cyclase in an on position. By binding to the membrane of enteric cells, cholera toxin stimulates the cellular adenylate cyclase system, causing the hyper-secretion of chloride and bicarbonate ions, which results in increased fluid secretion and the severe diarrhea characteristic of the disease cholera. The pentameric B subunit of CT binds with high efficiency to GM1 monosialoganglioside cell membrane receptors, present in many cell types, allowing its use experimentally in cell culture. Following binding, the toxin is endocytosed and travels retrogradely to the endoplasmic reticulum where the enzymatically active A subunit (CTA1) is translocated to the cytosol. Within the cytosol, CTA1 catalyzes the ADP-ribosylation of the Gs protein, activating adenylate cyclase and as a consequence increasing the intracellular concentration of cAMP. Relevant to the development of vaccine adjuvants, CT effectively modulates mammalian immune systems and acts as an adjuvant for co-administered antigens. Cholera Toxin from Vibrio cholerae, Inaba 569B Endotoxin: ~1EU/mg (Kinetic Chromagenic LAL assay) Activity: Using trisialoganglioside fixed sheep red blood cells, this lot exhibited strong hemagglutination activity when examined in a modification of Sato, et.et. Storage and Stability: Lyophilized powder may be stored at 4C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4C. Reconstituted product is stable for 6 months at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Reinheit: Purified
• Formulierung: Supplied as a lyophilized powder in 0.05M Tris, pH 7.5, 0.2M sodium chloride, 0.001M EDTA Na2. No preservative added. Reconstitute with 500ul sterile ddH2O. Handle Gently. Do not vortex.