A cell-permeable 1H-Pyrrole-2,3-dione derivative that covalently binds to RAD51 at Cys319 and irreversibly destabilizes a protein-protein interface that is essential for filament formation and recombinase activity. Stimulates single strand annealing at a 5-30uM concentration. Inhibits subnuclear RAD51 foci formation following DNA damage without affecting replication protein A. Also, effectively sensitizes cancer cells to mitomycin C (~25uM) and generate synergistic cell kill in RAD51C mutant cells and with lesser effect on RAD51 complemented cells. Satisfies all four Lipinski rules for predicting drug likeness. CAS No: 415713-60-9 Synonyms: 3-chloro-1-(3,4-dichlorophenyl)-4-morpholino-1H-pyrrole-2,5-dione Molecular Formula: C14H11Cl3N2O3 Molecular Weight: 361.6 Solubility: DMSO (50mg/ml) Appearance: Bright yellow solid. Purity: 99% (HPLC) Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
361.6
Reinheit:
99% (HPLC)
Formulierung:
Bright yellow solid.
CAS Nummer:
[415713-60-9]
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