Hepatitis A Virus (HAV) Antibody, IgM, BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-227698
Hersteller Artikelnummer:
227698
Alternativnummer:
USB-227698-48,USB-227698-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
The Hepatitis A Virus (HAV) Antibody, IgM, BioAssay(TM) ELISA Kit is for the qualitative determination of human anti-hepatitis A virus (HAV) antibody (IgM) concentrations in serum. Principle of the Assay: This ELISA Kit employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with anti-human IgM. Samples are pipetted into the wells and then human anti-HAV antibody (IgM) in samples is captured. After wash, add HAV antigen conjugated to Horseradish peroxidase (HRP) into the wells. Following a wash to remove any unbound reagent, a substrate solution is added to the wells. The color development is stopped and the intensity of the color is measured. Specificity: This assay has high sensitivity and excellent specificity for detection of human anti-HAV antibody (IgM). No significant cross-reactivity or interference between human anti-HAV antibody (IgM) and analogues was observed. Precision: Intra-assay Precision: CV <15% Inter-assay Precision: CV <15% Kit Components: 227698A: Microtiter Strips, 1x48 wells or 1x96 wells. Pre-coated, ready to use. 227698B: Negative Control, 1x500ul or 1x1ml 227698C: Positive Control, 1x500ul or 1x1ml 227698D: HRP Conjugate, 1x6ml or 1x12ml 227698E: Wash Buffer, 20X, 1x25ml or 1x50ml 227698F: Sample Diluent, 1x6ml or 1x12ml 227698G: Substrate A, 1x3ml or 1x6ml 227698H: Substrate B, 1x3ml or 1x6ml: 227698K: Stop Solution, 1x3ml or 1x6ml Storage and Stability: Store kit at 4C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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