Equine IgG (Immunoglobulin G) BioAssay(TM) ELISA Kit

Produktübersicht

• Artikelname: Equine IgG (Immunoglobulin G) BioAssay(TM) ELISA Kit
• Artikelnummer: USB-227732
• Hersteller Artikelnummer: 227732
• Alternativnummer: USB-227732-48,USB-227732-96
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The Equine IgG (Immunoglobulin G) BioAssay(TM) ELISA Kit is a competitive ELISA intended for the quantitative determination of IgG concentration in equine serum and plasma. Detection Range: 25-400ug/ml Sensitivity: <23.764ug/ml Precision: Intra-Assay CV: <8% Inter-Assay CV: <10% Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate has been pre-coated with equine IgG. Standards, samples and HRP-conjugated anti-equine IgG are added to the appropriate microtiter plate wells and a competitive reaction is launched between plate-coated equine IgG and equine IgG in the standards or samples for limited antibody binding sites on the HRP-conjugated anti-equine IgG reagent. The wells are washed to remove unbound materials. TMB substrate solution is then added to the wells, color development is stopped and absorbance read at 450nm wavelength. The intensity of the color is inversely proportional to the amount of equine IgG in the samples. Kit Components: 227665A: Microtiter Strips, 1x96 wells. Pre-coated, ready to use. 227665B: Standard, 0ug/ml, 1x500ul 227665C: Standard, 25ug/ml, 1x500ul 227665D: Standard, 50ug/ml, 1x500ul 227665E: Standard, 100ug/ml, 1x500ul 227665F: Standard, 200ug/ml, 1x500ul 227665G: Standard, 400ug/ml, 1x500ul 227665H: HRP Conjugate, 1x6ml 227665J: Sample Diluent 2x20ml 227665K: Wash Buffer, 25X, 1x20ml 227665L: TMB Substrate, 1x10ml 227665M: Stop Solution, 1x10ml Note: The Stop Solution (227732K) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store kit at 4C. The unopended kit is stable for 6 months after receipt. Once opened, the kit is stable up to 4 weeks at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Prepare reagents and samples as instructed. 2. Set a Blank well without any solution 3. Add 50ul Standard or sample to each well. 4. Add 50ul HRP Conjugate to each well (do not add any to the blank well). 5. Incubate for 30 minutes at 37C. 6. Aspirate and wash 5 times. 7. Add 90ul TMB Substrate to each well. 8. Incubate for 20 minutes at 37C. Protect from light 9. Add 50ul Stop Solution to each well. Read at 450nm within 5 minutes.