Testosterone (T), BioAssay(TM) ELISA Kit (Mouse)

Produktübersicht

• Artikelname: Testosterone (T), BioAssay(TM) ELISA Kit (Mouse)
• Artikelnummer: USB-227938
• Hersteller Artikelnummer: 227938
• Alternativnummer: USB-227938-48,USB-227938-96
• Hersteller: US Biological
• Kategorie: Kits/Assays
• Applikation: ELISA

Produktbeschreibung

Testosterone (T), BioAssay(TM) ELISA Kit (Mouse) is a competitive ELISA intended for the quantitative determination of endogenic mouse testosterone concentrations in serum, plasma and tissue homogenates. Detection Range: 0.1-20ng/ml Sensitivity: The minimum detectable dose of mouse testosterone is typically less than 0.05ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest mouse testosterone concentration that could be differentiated from zero. Specificity: This assay has high sensitivity and excellent specificity for detection of mouse Testosterone. No significant crossreactivity or interference between mouse testosterone and analogs was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the crossreactivity detection between mouse Testosterone and all the analogs. Therefore, crossreaction may still exist. Kit Components: 227938A: Microtiter Strips, 1x96 wells 227938B: Standard, 0ng/ml, 1x500ul 227938C: Standard, 0.1ng/ml, 1x500ul 227938D: Standard, 0.4ng/ml, 1x500ul 227938E: Standard, 1.6ng/ml, 1x500ul 227938F: Standard, 5ng/ml, 1x500ul 227938G: Standard, 20ng/ml, 1x500ul 227938H: Antibody 1x6ml 227938J: HRP Conjugate, 1x6ml 227938K: Wash Buffer, 20X, 1x15ml 227938L: Substrate A, 1x7ml 227938M: Substrate B, 1x7ml 227938N: Stop Solution, 1x7ml Note: The Stop Solution (227938N) provided with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material. Storage and Stability: Store all kit components at 4C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Assay Summary: 1. Prepare reagents and samples as instructed. 2. Set a Blank well without any solution 3. Add 50ul Standard or sample to each well. 4. Add 50ul HRP-conjugate to each well (Not to the Blank well). 5. Add 50ul Antibody to each well. 6. Incubate for 60 minutes at 37C. 7. Aspirate and wash 3 times. 8. Add 50ul Substrate A and 50ul Substrate B to each well. 9. Incubate for 15 minutes at 37C. Protect from light. 10. Add 50ul Stop Solution to each well. Read at 450nm within 10 minutes.