Neotetrazolium chloride is a tetrazolium dye that is used to detect the presence of living cells in a sample. It has an optimum concentration of 0.2% and significant cytotoxicity at concentrations greater than 1%. Neotetrazolium chloride binds to intracellular targets such as enzymes and nucleic acids, which can be detected by biochemical methods such as polymerase chain reaction (PCR). Neotetrazolium chloride can also bind to cell nuclei, making it useful for determining the number of viable cells in a sample. The bound form of neotetrazolium chloride is soluble in water and its detection limit is 10 fg/mL. Synonyms: 2,2,5,5-Tetraphenyl-3,3-[p-diphenylene] ditetrazolium chloride, 2-[4-(3,5-diphenyl-3H-tetraazol-2-ium-2-yl)[1,1-biphenyl]-4-yl]-3,5-diphenyl-3H-tetraazol-2-ium dichloride CAS No: 298-95-3 Molecular Formula: C38H28N82Cl Molecular Weight: 667.59 Appearance: Yellow to buff crystalline powderr Purity: 90% (HPLC) Melting Point: 228-234C Solubility: Methanol: 50mg/ml, Clear and complete solution Storage and Stability: May be stored at RT.
Molekulargewicht:
667.59
Reinheit:
90% (HPLC)
Formulierung:
Yellow to buff crystalline powder
CAS Nummer:
[298-95-3]
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