Poly(A)-binding Protein 1 (PABP1, PABP-1, PBP1, PAB1, PABP, PABPC1, PABPC2, PABPL1, Poly(A)-binding Protein Cytoplasmic 1, Polyadenylate Binding Protein 1), IgG2b, Clone: [10E10], Mouse, Monoclonal
Artikelnummer:
USB-254218
Hersteller Artikelnummer:
254218
Alternativnummer:
USB-254218-100
Hersteller:
US Biological
Wirt:
Mouse
Kategorie:
Antikörper
Applikation:
ELISA, FC, IF, IP, WB
Immunogen:
Fusion protein containing p53 sequence from amino acids 14-389
The poly(A)-binding protein (PABP) which is found complexed to the 3-prime poly(A) tail of eukaryotic mRNA is required for poly(A) shortening and translation initiation. Grange et al (1987) isolated a melanoma cell cDNA encoding human PABP. The predicted 633aa protein contains 4 repeats of an approximately 80aa unit in its N-terminal half. The authors found that this repeat region is highly conserved between human and yeast PABP and is sufficient for poly(A) binding. In vitro translation of the human PABP cDNA yielded a protein with an apparent molecular mass of 73kD by SDS-PAGE Northern blot analysis indicated that PABP is expressed as a 29-kb mRNA in human melanoma cells. Gorlach et al (1994) noted that each of the 4 repeats of PABP is a ribonucleoprotein (RNP) consensus sequence RNA-binding domain. They determined that PABP has a pI of approximately 103 and is a very abundant stable protein. Immunofluorescence studies of mammalian cells indicated that PABP is located exclusively in the cytoplasm. However using both indirect immunofluorescence and tagging of PABP1 by fusion to the green fluorescent protein (GFP). Afonina et al (1998) demonstrated that PABP1 shuttles between the nucleus and cytoplasm. PABP1 accumulated in the nucleus when transcription was inhibited suggesting that active transcription is required for nuclear export of PABP1. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: Use at an assay dependent dilution. Detects a band of ~70kD (predicted molecular weight: 71kD) Immunocytochemistry/Immunofluorescence: Use at an assay dependent dilution. PubMed: 17977970 Flow Cytometry: 1ug for 10e6 cells Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
