Coelenterazine, native (Luciferin, Preluciferin), CAS [[55779-48-1]]

Produktübersicht

• Artikelname: Coelenterazine, native (Luciferin, Preluciferin), CAS [[55779-48-1]]
• Artikelnummer: USB-257643
• Hersteller Artikelnummer: 257643
• Alternativnummer: USB-257643-5,USB-257643-10,USB-257643-25
• Hersteller: US Biological
• Kategorie: Biochemikalien

Produktbeschreibung

Coelenterazine is a luciferin, a light-emitting biomolecule that serves as a substrate for luciferases or as a constituent of photoproteins, including aequorin.1 The oxidation of coelenterazine to coelenteramide is accompanied by emission of blue light (emission maximum, 460-470 nm). Luciferase-mediated oxidation of coelenterazine or a derivative is used as an energy donor, typically to a form of green or yellow fluorescent protein, in bioluminescent resonance energy transfer studies.2,3 Alternatively, the calcium-mediated release of coelenterazine from aequorin, followed by non-enzymatic oxidation of this compound, results in bioluminescence. As light emission depends on both calcium and cellular redox status, this reaction is used to non-fluorescently detect changes in calcium level and redox status.4,5 Synonyms: 3,2-Dihydro-2-(p-hydroxybenzyl)-6-(p-hydroxyphenyl)-8-benzylimidazolo[1,2-a]pyrazin-3-one, CLZN, Preluciferin, Luciferin, 6-(4-hydroxyphenyl)-2-[(4-hydroxyphenyl)methyl]-8-(phenylmethyl)-imidazo[1,2-a]pyrazin-3(7H)-one CAS No: 55779-48-1 Molecular Formula: C26H21N3O3 Molecular Weight: 423.46 Appearance: Brownish/yellow to yellow/orange crystalline powder Purity: 95% Solubility (0.0125mg/ml in methanol: Orange to yellow to brown liquid Molar Extinction Coefficient (0.0125mg/ml in methanol, lambda max1): >7800.0 L/(mol cm) UV (0.0125mg/ml in methanol, lambda max1): 431-435nm UV (0.0125mg/ml in methanol, lambda max2): 261-265nm Storage and Stability: Store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Hygroscopic and light sensitive. Store in amber vial under inert atmosphere. Note: It is not recommended to store dissolved coelenterazine at -20C or -70C. Coelenterazine is a high energy dioxetanone ring structure that will spontaneously decompose even at low temperatures.

Produkteigenschaften

• Molekulargewicht: 423.46
• Reinheit: 95%
• Formulierung: Brownish/yellow to yellow/orange crystalline powder
• CAS Nummer: [55779-48-1]