Nitrocefin is a chromogenic beta-lactamase substrate that undergoes colour change from yellow to red as the amide bond in the beta-Lactam ring is hydrolyzed by beta-lactamase. Nitrocefin undergoes colour changes induced by lactamases produced by Gram-positive and Gram-negative bacteria. Several studies have utilized the colour changing properties of Nitrocefin for the detection of beta-lactamase activity from bacterial cell extracts by isoelectric focusing and spectroscopy. Nitrocefin has also been used in studies involving beta-lactamase resistant antibiotics. Synonyms: (6R,7R)-3-[(1E)-2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid, [6R-[3(E),6alpha,7beta]]-3-[2-(2,4-dinitrophenyl)ethenyl]-8-oxo-7-[(2-thienylacetyl)amino]-5-thia-1-azabicyclo[4.2.0]oct-2-ene-2-carboxylic Acid CAS No: 41906-86-9 Molecular Formula: C21H16N4O8S2 Molecular Weight: 516.50 Purity: 98% Appearance: Orange to red solid Solubility: DMF: 20 mg/ml, DMSO: 20 mg/ml Melting Point: 103-113C (dec.) (lit) Storage and Stability: May be stored at RT for short-term only. Long-term storage is recommended at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molekulargewicht:
516.5
CAS Nummer:
[41906-86-9]
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