Mutanolysin has been used in a study to assess lysing and generating protoplasts of dairy streptococci. It has also been used in a study to investigate the conversion of group B streptococci to protoplasts. It provides gentle cell lysis for the isolation of easily degradable biomolecules and RNA from bacteria. It has been used in the formation of spheroplasts for isolation of DNA. Mutanolysin is an N-acetylmuramidase. Like lysozyme, it is a muralytic enzyme that cleaves the beta-N-acetylmuramyl-(14)-N-acetylglucosamine linkage of the bacterial cell wall polymer peptidoglycan-polysaccharide. Its carboxy terminal moieties are involved in the recognition and binding of unique cell wall polymers. Mutanolysin lyses Listeria and other Gram-positive bacteria such as Lactobacillus and Lactococcus. Source: Streptomyces globisporus CAS No: 55466-22-3 Molecular Weight: 23kD Purity: 4000units/mg Appearance: White to off-white powder containing Ficoll and sodium succinate buffer salts. Protein: 4000units/mg Protein (Biuret): 40% Unit Definition: One unit will produce a deltaA600 of 0.01 per minute at pH 6.0 at 37C in a 1ml volume using a suspension of Streptococcus faecalis cell wall as substrate. Storage and Stability: Store at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Molekulargewicht:
23
CAS Nummer:
[55466-22-3]
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