BRD1 Inhibitor Screening, BioAssay(TM) Kit (Bromodomain Testis-Specific Protein, BRDT, BRD6, CT9)

Produktübersicht

• Artikelname: BRD1 Inhibitor Screening, BioAssay(TM) Kit (Bromodomain Testis-Specific Protein, BRDT, BRD6, CT9)
• Artikelnummer: USB-298338
• Hersteller Artikelnummer: 298338
• Alternativnummer: USB-298338-1
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The BRD1 Inhibitor Screening BioAssay(TM) Kit is designed to measure the inhibition of BRD1 binding to its substrate. The key to the BRD1 Inhibitor Screening BioAssay(TM) Kit is the specific binding of the BRD1 bromodomain to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD1 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts. Applications: Suitable for use in the study of bromodomain binding assays, screening inhibitors and selectivity profiling. Other applications not tested. Kit Components: BRD1 (561-668), GST-tag, 1x24ug BET Ligand 1, 1x400ul Non-acetylated BET Ligand 1, 1x200ul 3x BRD Homogeneous Assay Buffer 2, 1x4ml 3x BRD Homogeneous Detection Buffer 2, 1x3ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.