BRD2 Inhibitor Screening, BioAssay(TM) Kit (BD2, RNF3, RING3, Bromodomain)

Produktübersicht

• Artikelname: BRD2 Inhibitor Screening, BioAssay(TM) Kit (BD2, RNF3, RING3, Bromodomain)
• Artikelnummer: USB-298348
• Hersteller Artikelnummer: 298348
• Alternativnummer: USB-298348-1
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

The BRD2 (BD2) Inhibitor Screening BioAssay(TM) Kit is designed to measure the inhibition of BRD2 bromodomain 2 (BD2) from binding to its substrate. The key to the BRD2 (BD2) Inhibitor Screening BioAssay(TM) Kit is the highly specific binding of the BRD2 bromodomain 2 to the acetylated histone substrate. With this kit, only three simple steps on a microtiter plate are required. First, a sample containing BRD2 bromodomain 2 and an inhibitor of choice is incubated with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then donor beads, followed by reading the Alpha-counts. Applications: Suitable for use in the study of bromodomain binding assays, screening inhibitors and selectivity profiling. Other applications not tested. Kit Components: BRD2 (339-459), BD2, GST-tag, 1x20ug BET Bromodomain Ligand 1x400ul Non-acetylated Ligand 1, 1x200ul 3x BRD Homogeneous Assay Buffer 1, 1x4ml 3x BRD Homogeneous Detection Buffer 1, 1x3ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.