Phosphate, PBlue, BioAssay(TM) Kit

Produktübersicht

• Artikelname: Phosphate, PBlue, BioAssay(TM) Kit
• Artikelnummer: USB-299029
• Hersteller Artikelnummer: 299029
• Alternativnummer: USB-299029-500
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

Intended Use: Phosphatase Assays: liberation of phosphate from peptide, protein or small molecule substrate. Lipase Assays: liberation of phosphate from phospholipids Nucleoside Triphosphatase Assays: liberation of phosphate from nucleoside triphosphates (ATP, GTP, TTP, CTP etc). Quantitation of Phosphate: in phospholipids, proteins and DNAs, etc. Drug Discovery: high-throughput screen for phosphatase inhibitors. Sensitivity: Detection of as little of 20pmoles of phosphate Range: 0.4-50uM Test Principle: The PBlue Phosphate BioAssay(TM) Kit is based on a proprietary formulation of the malachite green dye. The PBlue reagent forms a blue colored complex with free orthophosphate. The rapid color formation from the reaction can be conveniently measured on a spectrophotometer (600-660nm) or on a plate reader. The non-radioactive colorimetric assay kits have been optimized to offer superior sensitivity and prolonged shelf life. The assay is simple and fast, involving a single addition step for phosphate determination. Assays can be performed in tubes, cuvettes or multi-well plates. The assays can be conveniently executed in 96-well plates for high throughput screening of enzyme inhibitors. Kit Components: Reagent, 1x50ml Standard, 1mM phosphate, 1x1ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.