Leukemia Inhibitory Factor, Recombinant, Rat (LIF)

Produktübersicht

• Artikelname: Leukemia Inhibitory Factor, Recombinant, Rat (LIF)
• Artikelnummer: USB-299441
• Hersteller Artikelnummer: 299441
• Alternativnummer: USB-299441-10
• Hersteller: US Biological
• Kategorie: Molekularbiologie

Produktbeschreibung

Leukemia Inhibitory Factor (LIF) is a lymphoid factor which promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Rat LIF (rtLIF) is a 19.9kD protein containing 183aa residues that exhibits 91% aa sequence identity with murine LIF1. Analysis of the secondary structure of rat and murine LIF indicates a difference in the two dimensional structure of both proteins. Studies have shown that rtLIF is more effective in maintaining the undifferentiated phenotype of rat ES cells than similar concentrations of murine LIF Source: Recombinant protein corresponding to 183aa of rat LIF, expressed in E. coli. Molecular Weight: ~19.9kD Biological Activity: Determined by the ability to induce differentiation of M1 myeloid leukemic cells. The minimum detectable concentration of rat LIF in this assay is 0.5ng/ml. Specific Activity: >1x10e8units/mg Unit Definition: 50 units is defined as the amount of rat LIF required to induce differentiation in 50% of the M1 colonies in 1ml agar cultures. Endotoxin: <0.1ng/ug Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Produkteigenschaften

• Molekulargewicht: 19.9
• Reinheit: ~85% (HPLC, SDS-PAGE)
• Formulierung: Supplied as a liquid in 50mM sodium phosphate, pH 7.4, 1mM DTT, 250mM sodium chloride, 0.02% Tween 20, 10% glycerol. No preservative added.