Antibody Rapid Labeling, Conjugation Kit, MaxLight(TM) 490

Produktübersicht

• Artikelname: Antibody Rapid Labeling, Conjugation Kit, MaxLight(TM) 490
• Artikelnummer: USB-301154
• Hersteller Artikelnummer: 301154
• Alternativnummer: USB-301154-3X100
• Hersteller: US Biological
• Kategorie: Kits/Assays

Produktbeschreibung

MaxLight(TM) Rapid Labeling Kits Titered for ease of use Ready to label 3x100ug conjugations/kit 100ug of antibody at 0.9-1.0mg/ml Complete and purified conjugation in 1hour with 15 hands on time: >90% yield Everything included Steps: spin, transfer, incubate, spin, spin, done. No buffers, no organic solvents, no calculations Simple protocol using just a microcentrifuge and a Nanodrop or UV spec Incorporates the optimal number of dye per antibody Pure product is produced, all excess label is removed. Exclusive to MaxLight(TM) kits Multiple antibodies can be modified simultaneously with different labels Simple Protocol: 1. Prepare Antibody (~100ug ~1mg/ml) 2. Prepare Desalting Column (Blue Cap), add antibody, centrifuge (2min) 3. Add Antibody to Labeling Column (Clear Cap), vortex, incubate (1hr, dark, RT) 4. Prepare 2 Purification Columns (Red Cap), add labeled antibody, centrifuge (2min) 5. Repeat (add antibody to Purification Column 2 (Red Cap), centrifuge (2min) 6. Flow-through is final labeled antibody, free of unconjugated dye. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Now Available: MaxLight(TM) Biotin (PEG4) MaxLight(TM) HRP MaxLight(TM) FITC MaxLight(TM)405 (Violet: compare to AlexaFluor405, PacificBlue(TM), Brilliant(TM) Violet 421) MaxLight(TM)490 (Blue-Green: compare to DyLight(TM)488, AlexaFluor488) MaxLight(TM)550 (Yellow-Green: compare to AlexaFluor546, 555, DyLight(TM)549 , Cy3(TM), TRITC) MaxLight(TM)650 (Far-IR: compare to AlexaFluor647, DyLight(TM)649, Cy5(TM)) MaxLight(TM)750 (Near IR: compare to DyLight(TM)755) Specifications: Abs Em epsilon MaxLight(TM) Biotin - - - MaxLight(TM) HRP - - - MaxLight(TM) FITC 494 520 73,000 MaxLight(TM)405 400 423 32,000 MaxLight(TM)490 493 518 70,000 MaxLight(TM)550 550 568 150,000 MaxLight(TM)650 646 674 120,000 MaxLight(TM)750 655 676 250,000