Residues aa254-270 [RATEKFQLAVEALEQEK] of 177kD human Nestin protein.
Nestin is an intermediate filament protein that is expressed predominantly in stem cells of the central nervous system in the neural tube. Upon differentiation, Nestin is replaced by cell type-specific intermediate filaments, (e.g., neurofilaments and glial fibrillary acidic protein (GFAP)). Nestin expression is re-induced after CNS injury and during regeneration of injured muscle tissue. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:200-1:500 Western Blot: 1:500-1:1000 Immunohistochemistry: 1:100-1:200 Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Reinheit:
Purified by immunoaffinity chromatography.
Formulierung:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
