Orexin-A and B were identified as endogenous ligands for the orexin-1 (HCRT1) and orexin-2 (HCRT2) G-protein coupled receptors (Sakurai et al, 1998). These peptides are identical to two hypothalamic peptides that share a high degree of homology with secretin, designated hypocretin-1 and hypocretin-2 (De Lecea et al, 1998). ICV administration of orexin-A and B stimulated food intake in rats. Additionally, prepro-orexin mRNA levels were upregulated in fasting rats. Recent reports suggest a role in the sleep-wake cycle. Molecular Weight: ~4033.70 Sequence: Leu-Gly-Val-Asp-Ala-Gln-Pro-Leu-Pro-Asp-Cys-Cys-Arg-Gln-Lys-Thr-Cys-Ser-Cys-Arg-Leu-Tyr-Glu- Leu-Leu-His-Gly-Ala-Gly-Asn-His-Ala-Ala-Gly-Ile-Leu-Thr-Leu-NH2, Disulfide Bridge: Cys11-Cys17 and Cys12-Cys19 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molekulargewicht:
4033.7
Reinheit:
~97% (HPLC)
Formulierung:
Supplied as a white powder.
* Mehrwertsteuer und Versandkosten nicht enthalten. Irrtümer und Preisänderungen vorbehalten
