Annexin V (Anchorin CII, Annexin 5, Annexin A5, Annexin-5, Annexin5, AnnexinA5, AnnexinV, ANX 5, ANX A5, ANX5, ANXA5, ANXA5_HUMAN, Calphobindin I, CBP I, CBP-I, Endonexin II, ENX 2, ENX2, Lipocortin V, PAP I, PAP-I, Placental Anti
Artikelnummer:
USB-303117
Hersteller Artikelnummer:
303117
Alternativnummer:
USB-303117-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Recombinant protein corresponding to human Annexin V expressed in E.coli. (Position: A2-D320). Species sequence homology: mouse (94%) and rat (92%)
Annexin A5 (or annexin V) is a cellular protein in the annexin group. It is mapped to 4q27. Annexin A5 has been proposed to play a role in the inhibition of blood coagulation by competing for phosphatidylserine binding sites with prothrombin and also to inhibit the activity of phospholipase A1. This protein is an anticoagulant protein, it can form a shield around negatively-charged phospholipid molecules, the formation of an annexin A5 shield blocks the entry of phospholipids into coagulation (clotting) reactions. And Annexin A5 can also act as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Immunohistochemistry (frozen): 0.5-1ug/ml Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
