APE (AP Endonuclease 1, AP Endonuclease Class I, AP Lyase, APE 1, APE1, APE-1, APEN, APEX 1, APEX, APEX Nuclease (Multifunctional DNA Repair Enzyme) 1, Apex Nuclease 1, Apex Nuclease, APEX1, APEX1_HUMAN, Apurinic Endonuclease, Apu
APE (AP Endonuclease 1, AP Endonuclease Class I, AP Lyase, APE 1, APE1, APE-1, APEN, APEX 1, APEX, APEX Nuclease (Multifunctional DNA Repair Enzyme) 1, Apex Nuclease 1, Apex Nuclease, APEX1, APEX1_HUMAN, Apurinic Endonuclease, Apu
APE (AP Endonuclease 1, AP Endonuclease Class I, AP Lyase, APE 1, APE1, APE-1, APEN, APEX 1, APEX, APEX Nuclease (Multifunctional DNA Repair Enzyme) 1, Apex Nuclease 1, Apex Nuclease, APEX1, APEX1_HUMAN, Apurinic Endonuclease, Apu
Artikelnummer:
USB-303121
Hersteller Artikelnummer:
303121
Alternativnummer:
USB-303121-100
Hersteller:
US Biological
Wirt:
Rabbit
Kategorie:
Antikörper
Applikation:
IHC, WB
Immunogen:
Recombinant protein corresponding to human APE expressed in E. coli. (Position: P2-L318). Species sequence homology: mouse (94%) and rat (93%)
APEX1, also called apurinic endonuclease (APE), is a DNA repair enzyme having apurinic/apyrimidinic (AP) endonuclease, 3-prime, 5-prime-exonuclease, DNA 3-prime repair diesterase, and DNA 3-prime-phosphatase activities. The human APEX1 gene consists of 5 exons spanning 2.64 kb and exists as a single copy in the haploid genome. Using in situ hybridization, the APEX1 gene is mapped to 14q11.2-q12. The predicted APEX1 protein, which contained probable nuclear transport signals, was identified as a member of a family of DNA repair enzymes found in lower organisms. The abundance of the large form of APEX1 was increased in leiomyoma extracts relative to myometrial tissue extracts, and the large form was dominant in cell lines derived from leiomyosarcomas. The exonuclease activity of nuclear APEX1 can remove the anti-HIV nucleoside analogs AZT and D4T from the 3-prime terminus of a nick more efficiently than can cytosolic exonucleases. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions. Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
