Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Partial recombinant protein corresponding to aa113-261 from human CD40 Ligand, Tag-free expressed in E. coli. Swiss/UniProt Accession: P29965. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of IL-8 production by human PBMC is less than 5-10ng/ml. Amino Acid Sequence: MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LWLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL Molecular Weight: 16.3kD PubMed ID: 1280226, 1385114, 7678782, 1281209, 7678552, 7999797, 15489334, 8626375, 11676606, 15193700, 8589998, 9605317, 7679206, 8094231, 7679801, 7717401, 7532185, 8889581, 8550833, 9150729, 9746782 Gene Name: CD40LG, CD40L, TNFSF5, TRAP Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
