The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium (By similarity). Binds to CX3CR1. {ECO:0000250}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human peripheral blood lymphocytes (PBL) is less than 0.5ug/ml, corresponding to a specific activity of > 2000 IU/mg. Sequence: QHLGMTKCEI MCGKMTSRIP VALLIRYQLN QESCGKRAIV LETTQHRRFC ADPKEKWVQD AMKHLDHQAA ALTKNG Molecular Weight: 8.7kD PubMed ID: 9177350, 9479488, 10e382755, 16141072, 15489334, 9845323, 21183079 Gene Name: Cx3cl1,Cx3c,Fkn,Scyd1 Swiss Prot: O35188 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
