| The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development., Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using serum free human MCF-7 cells is less than 2ng/ml, corresponding to a specific activity of > 5.0x10e5 IU/mg. Sequence: AYRPSETLCG GELVDTLQFV CGDRGFYFSR PASRVSRRSR GIVEECCFRS CDLALLETYC ATPAKSE Molecular Weight: 7.5kD PubMed ID: 6382022, 6382021, 2450353, 3002851, 3476948, 3683205, 3881277, 7730145, 16531418, 20842449, 15498874, 14702039, 16554811, 15489334, 3167054, 3653397, 3652904, 658418, 2722836, 7633596, 11448941, 12586351, 15359740, 1569071, 16912056, 19066168, 19838169, 22171320, 23234360, 6189745, 7527339, 18046459 Gene Name: IGF2,PP1446 Swiss Prot: P01344 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
