| Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. {ECO:0000269|PubMed:3920526}. Full-lenth recombinant protein corresponding to aa117-269 from human mature IL1B, expressed in E. coli. Swiss/UniProt Accession: P01584. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine D10S cells is less than 1.0pg/ml, corresponding to a specific activity of >1.0x10e9 IU/mg. Amino Acid Sequence: APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIP VAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQ FPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS Molecular Weight: 17.3kD PubMed ID: 6083565, 2989698, 3490654, 3493774, 2954882, 2635664, 11991722, 15815621, 15489334, 1919436, 3920526, 3281727, 1837236, 17431422, 3259176, 2585509, 2602367, 2372550, 2001363, 9062193, 20802483 Gene Name: IL1B,IL1F2 Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O, 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
