| Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2, however, the physiological relevance of the latter association is unsure. {ECO:0000269|PubMed:7775431}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by inhibiting IL-1alpha- dependent proliferation of murine D10S cells is less than 40ng/ml, corresponding to a specific activity of > 2.5x10e4 IU/mg in the presence of 50 pg/ml rHuIL-1alpha. Sequence: RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE Molecular Weight: 17.1kD PubMed ID: 2139180, 2137201, 1828896, 1385987, 8992991, 1827201, 7629520, 14702039, 15815621, 15489334, 2137200, 2143761, 9514884, 7989776, 7775431, 11991722, 8786086, 16335952, 19494218, 1534997, 8045306, 8175703, 7867645, 9062194, 18987736 Gene Name: IL1RN, IL1F3, IL1RA Swiss Prot: P18510 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
