| IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus. IL-8(6-77) has a 5-10-fold higher activity on neutrophil activation, IL-8(5-77) has increased activity on neutrophil activation and IL-8(7-77) has a higher affinity to receptors CXCR1 and CXCR2 as compared to IL-8(1-77), respectively. {ECO:0000269|PubMed:11978786, ECO:0000269|PubMed:2145175, ECO:0000269|PubMed:2212672}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human CXCR2 transfected mouse BaF3 cells is less than 2ng/ml, corresponding to a specific activity of > 5.0 *10e5 IU/mg. Sequence: SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS Molecular Weight: 8.4kD PubMed ID: 2953813, 3260265, 2663993, 2664463, 2200751, 14702039, 15489334, 2523801, 2145175, 2648135, 2659722, 1755384, 2655583, 8344717, 3279957, 3480540, 3322281, 2212672, 2007144, 11023497, 11978786, 1639201, 14711052, 18710930, 19608678, 20829347, 2681204, 2184886, 8631339, 10e368283, 2182630, 2005614, 1988949, 10707023 Gene Name: CXCL8,IL8 Swiss Prot: P10145 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
