Chemotactic activity for lymphocytes but not for monocytes or neutrophils. {ECO:0000250}. Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human XCR1 transfected murine BaF3 cells is less than 100ng/ml, corresponding to a specific activity of >1*10e4 IU/mg. Sequence: VGTEVLQESI CVSLRTQRLP VQKIKTYTIK EGAMRAVIFV TKRGLRICAD PQAKWVKTAI KTVDGRASAS KSKAETIPTQ AQRSASTAVT LTG Molecular Weight: 10.0kD Gene Name: Xcl1, Ltn, Scyc1 Swiss Prot: P51672 Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
