AuH, also known as mitochondrial methylglutaconyl-CoA hydratase, is involved in the aa degradation pathway by catalyzing the conversion of 3-methylglutaconyl-CoA to 3-hydroxy-3- methylglutaryl-CoA and water. It has been found to have very low enoyl-CoA hydratase activity. Source: Recombinant protein corresponding to aa68-339 of human AuH, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~31.4kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSSEMKTEDE LRVRHLEEEN RGIVVLGINR AYGKNSLSKN LIKMLSKAVD ALKSDKKVRT IIIRSEVPGI FCAGADLKER AKMSSSEVGP FVSKIRAVIN DIANLPVPTI AAIDGLALGG GLELALACDI RVAASSAKMG LVETKLAIIP GGGGTQRLPR AIGMSLAKEL IFSARVLDGK EAKAVGLISH VLEQNQEGDA AYRKALDLAR EFLPQGPVAM RVAKLAINQG MEVDLVTGLA IEEACYAQTI PTKDRLEGLL AFKEKRPPRY KGE Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
