CoaA, Recombinant, E. coli, aa1-316, His-Tag (Pantothenate kinase)
Artikelnummer:
USB-349805
Hersteller Artikelnummer:
349805
Alternativnummer:
USB-349805-100,USB-349805-500
Hersteller:
US Biological
Kategorie:
Molekularbiologie
coaA (pantothenate kinase) belongs to the prokaryotic pantothenate kinase family. Pantothenate kinase (PanK, CoaA) is the first enzyme in the Coenzyme A biosynthetic pathway. It phophorylates pantothenate (vitamin B5) to form 4-phosphopantothenate. Regulation of pantothenate kinase activity by feedback inhibition is the critical factor controlling the intracellular CoA concentration. Source: Recombinant protein corresponding to aa1-316 of E. coli coaa, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~38.9kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMSIKEQ TLMTPYLQFD RNQWAALRDS VPMTLSEDEI ARLKGINEDL SLEEVAEIYL PLSRLLNFYI SSNLRRQAVL EQFLGTNGQR IPYIISIAGS VAVGKSTTAR VLQALLSRWP EHRRVELITT DGFLHPNQVL KERGLMKKKG FPESYDMHRL VKFVSDLKSG VPNVTAPVYS HLIYDVIPDG DKTVVQPDIL ILEGLNVLQS GMDYPHDPHH VFVSDFVDFS IYVDAPEDLL QTWYINRFLK FREGAFTDPD SYFHNYAKLT KEEAIKTAMT LWKEINWLNL KQNILPTRER ASLILTKSAN HAVEEVRLRK Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
