NEIL1 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction. Recombinant protein corresponding to aa1-390 of human NEIL1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~45.8kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MPEGPELHLA SQFVNEACRA LVFGGCVEKS EPLALVFRFG MSGSFQLVPR EELPRHAHLR FYTAPPGPRL ALCFVDIRRF ICEALLDQRF FNGIGNYLRA EILYRLKIPP FEKARSVLEA LQQHRPSPEL EDFAAFRAWL RCYGMPGMSS LQDRHGRTIW FQGDPGPLAP KGRKSRKKKS EGTSLQQDPE APTVPKKGRR KGRQAASGHC RPRKVKADIP SLEPEGTSAS SVSRNPEVPF ESSAYRISAS ARGKELRLIL SPLPGAQPQQ GRWDLGGKWQ PGRGPCVLQE YQQFRENVLR NLADKAFDRP TLSQKIRTKL QNPDLLELCH SVPKEVVQLG GKGYGSESGE KATQLSPEDR VEDALPPSKA PSRTRRAKRD LPKRTATQRP Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
