NEK7 is protein kinase which plays an important role in mitotic cell cycle progression. It is required for microtubule nucleation activity of the centrosome, robust mitotic spindle formation and cytokinesis. NEK7 is highly expressed in lung, muscle, testis, brain, heart, liver, leukocyte and spleen. Source: Recombinant protein corresponding to aa1-302 of human NEK7, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~37kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMDEQSQ GMQGPPVPQF QPQKALRPDM GYNTLANFRI EKKIGRGQFS EVYRAACLLD GVPVALKKVQ IFDLMDAKAR ADCIKEIDLL KQLNHPNVIK YYASFIEDNE LNIVLELADA GDLSRMIKHF KKQKRLIPER TVWKYFVQLC SALEHMHSRR VMHRDIKPAN VFITATGVVK LGDLGLGRFF SSKTTAAHSL VGTPYYMSPE RIHENGYNFK SDIWSLGCLL YEMAALQSPF YGDKMNLYSL CKKIEQCDYP PLPSDHYSEE LRQLVNMCIN PDPEKRPDVT YVYDVAKRMH ACTASS Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
