Non-homologous end-joining factor 1, also known as NHEJ1, belongs to the XLF family. NHEJ1 was originally discovered as the protein mutated in five patients with growth retardation, microcephaly, and immunodeficiency. The protein is required for the non-homologous end joining (NHEJ) pathway of DNA repair. Patients with NHEJ1 mutations also have immunodeficiency due to a defect in V(D)J recombination, which utilizes NHEJ to promote immune system diversity. XLF interacts with DNA ligase IV and XRCC4 and is thought to be involved in the ligation step of NHEJ. Source: Recombinant protein corresponding to aa1-224 of human NHEJ1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~27.8kD AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEELEQG LLMQPWAWLQ LAENSLLAKV FITKQGYALL VSDLQQVWHE QVDTSVVSQR AKELNKRLTA PPAAFLCHLD NLLRPLLKDA AHPSEATFSC DCVADALILR VRSELSGLPF YWNFHCMLAS PSLVSQHLIR PLMGMSLALQ CQVRELATLL HMKDLEIQDY QESGATLIRD RLKTEPFEEN SFLEQFMIEK LPEACSIGDG KPFVMNLQDL YMAVTTQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
