Deoxyribonucleoside 5-monophosphate N-glycosidase isoform 1, also known as RCL, belonged to the N-deoxyribosyltransferase family. This protein catalyzes the cleavage of the N-glycosidic bond of deoxyribonucleoside 5-monophosphates to yield deoxyribose 5-phosphate and a purine or pyrimidine base. RCL was identified on the basis of its stimulation by c-Myc protein. The latter is a transcription factor that participates in the regulation of cell proliferation, differentiation, and apoptosis. Source: Recombinant protein corresponding to aa1-174 of human RCL, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~21.2kD Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAAAMVPGRS ESWERGEPGR PALYFCGSIR GGREDRTLYE RIVSRLRRFG TVLTEHVAAA ELGARGEEAA GGDRLIHEQD LEWLQQADVV VAEVTQPSLG VGYELGRAVA FNKRILCLFR PQSGRVLSAM IRGAADGSRF QVWDYEEGEV EALLDRYFEA DPPGQVAASP DPTT Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
