ARL9 (ADP-ribosylation factor-like protein 9) belongs to two novel subfamilies coding for small GTPases that we found to be highly conserved among eukaryotes. Recombinant human ARL9 protein, fused to His-tag at N-terminus, expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer. Recombinant protein corresponding to aa1-123 from human ARL9, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~15.9kD (143aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MEFLEIGGSK PFRSYWEMYL SKGLLLIFVV DSADHSRLPE AKKYLHQLIA ANPVLPLVVF ANKQDLEAAY HITDIHEALA LSEVGNDRKM FLFGTYLTKN GSEIPSTMQD AKDLIAQLAA DVQ Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
