BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant protein corresponding to aa28-392 from human BCAT2, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~43.9kD (390aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASSSF SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL SQPTRALLFV ILCPVGAYFP GGSVTPVSLL YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL ENGPELILRF QKELKEIQYG IRAHEWMFPV KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
