Glk, Recombinant, E. coli, aa1-321, His-Tag (Glucokinase)
Artikelnummer:
USB-351246
Hersteller Artikelnummer:
351246
Alternativnummer:
USB-351246-20,USB-351246-100
Hersteller:
US Biological
Kategorie:
Molekularbiologie
Glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Source: Recombinant protein corresponding to aa1-321 from E. coli glk, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~37.1kD (344aa) Specific Activity: >70units/mg, obtained by measuring the increase of NADPH in absorbance at 340nm resulting from the reduction of NADP. One unit will oxidize 1umole of Glucose to D-glucose 6-phosphate/minute in the presence of Beta-NADP at pH 9.0 at 37C. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN RLPENLKPKD ITERALADSC TDCRRALSLF CVIMGRFGGN LALNLGTFGG VFIAGGIVPR FLEFFKASGF RAAFEDKGRF KEYVHDIPVY LIVHDNPGLL GSGAHLRQTL GHIL Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
