Glutathione S-transferase mu 5, also known as GSTM5, is member of the glutathione s-transferase (GST) family of proteins. There are eight families of GST proteins, namely alpha, kappa, mu, omega, pi, sigma, theta and zeta, each of which is composed of proteins that have a variety of functions throughout the cell. GSTM5 plays an important role in detoxification. Source: Recombinant protein corresponding to aa1-218 from human GSTM5, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~28.2kD (242aa) Specific Activity: ~90,000 pmol/min/ug, and is defined as the amount of enzyme that conjugate 1umole of 1-chloro-2,4- dinitrobenzene (CDNB) with reduced glutathione/minute at pH 6.5 at 25C. Activity Assay: 1. Prepare a 180ul assay buffer into a suitable container. The concentrations are 106mM potassium phosphate (pH6.5), 1.1mM EDTA, 5.5mM glutathione, reduced, 2.2mM 1-chloro-2,4-dinitrobenzene (CDNB), 8% (v/v) ethanol. 2. Equilibrate to 25C and monitor at A340nm until the value is constant using a spectrophotometer. 3. Add 20ul of recombinant GSTM5 protein 1.25ug/ml in assay buffer. 4. Mix by inversion and record the decrease at A340nm for approximately 5 minutes. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMPMTLG YWDIRGLAHA IRLLLEYTDS SYVEKKYTLG DAPDYDRSQW LNEKFKLGLD FPNLPYLIDG AHKITQSNAI LRYIARKHNL CGETEEEKIR VDILENQVMD NHMELVRLCY DPDFEKLKPK YLEELPEKLK LYSEFLGKRP WFAGDKITFV DFLAYDVLDM KRIFEPKCLD AFLNLKDFIS RFEGLKKISA YMKSSQFLRG LLFGKSATWN SK Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
