NUDT3, also known as DIPP, DIPP1 (diphosphoinositol polyphosphate phosphohydrolase 1), is a 172aa cytoplasmic protein belonging to the nudix hydrolase family and DIPP subfamily. NUDT3 acts as a negative regulator of the ERK 1/2 pathway and hydrolyzes 5-phosphoribose 1-diphosphate. NUDT3 exists as a monomer and binds magnesium as a cofactor. Also NUDT3 is widely expressed but found at highest levels in liver, pancreas, brain and heart. Source: Recombinant protein corresponding to aa1-172 from human NuDT3, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~21.6kD (192aa) AA Sequence: MGSSHHHHHH SSGLVPRGSH MMKLKSNQTR TYDGDGYKKR AACLCFRSES EEEVLLVSSS RHPDRWIVPG GGMEPEEEPS VAAVREVCEE AGVKGTLGRL VGIFENQERK HRTYVYVLIV TEVLEDWEDS VNIGRKREWF KIEDAIKVLQ YHKPVQASYF ETLRQGYSAN NGTPVVATTY SVSAQSSMSG IR Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
