SMS (Spermine synthase) belongs to the spermidine/spermine synthase family. It is an enzyme that converts spermidine into spermine. This enzyme is required for normal viability, growth and fertility involved in polyamine metabolism. Defects in SMS are the cause of Snyder-Robinson syndrome (SRS), also known as X- linked mental retardation Snyder-Robinson type. SRS is characterized by moderate intellectual deficit, hypotonia, an unsteady gait, osteoporosis, kyphoscoliosis and facial asymmetry. Transmission is X-linked recessive. Recombinant protein corresponding to aa1-366 from human SMS, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~43.8kD (390aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMAAARH GLVLLDLQSY DGDAQGKEEI DSILNKVEER QFGNILILSG DVNLAESDLA YTRAIMGSGK DCYQVLIEDC IPVLKRYAKE GREFDYVIND CPVEFSKEIV CVPSYLELWV FYTVWKKAKP STLDFMLGAK ADGETILKGL QSIFQEQGMA ESVHTWQDHG YLATYTNKNG SFANLRIYPH MKELSQDSTG RVKRLPPIVR GGAIDRYWPT ADGRLVEYDI DEVVYDEDSP YQNIKILHSK EDYTGKDVLI LGGGDGGILC EIVKLKPKMV TMVEIDQMVI DGCKKYMRKT CGDVLDNLKG LTAVPISTSP EEDSTWEFLR LILDLSMKVL KQDGKYFTQG NCVNLTEALS LYEEQLGRLY Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
