Uridine phosphorylase 1, also known as UPP1 catalyses the reversible phosphorolysis of uridine to uracil. The reaction products are then utilized as carbon and energy sources, or in the rescue of pyrimidine bases for nucleotide synthesis. The expression levels and the enzymatic activity of UPP1 are higher in human solid tumors than in adjacent normal tissues. In addition, UPP1 controls the homeostatic regulation of uridine concentration in plasma and tissues and plays a role in the intracellular activation of 5-fluorouracil. Recombinant protein corresponding to aa1-310 from human uPP1, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight: ~36kD (330aa) Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG SPSRMKAFIR CVGAELGLDC PGRDYPNICA GTDRYAMYKV GPVLSVSHGM GIPSISIMLH ELIKLLYYAR CSNVTIIRIG TSGGIGLEPG TVVITEQAVD TCFKAEFEQI VLGKRVIRKT DLNKKLVQEL LLCSAELSEF TTVVGNTMCT LDFYEGQGRL DGALCSYTEK DKQAYLEAAY AAGVRNIEME SSVFAAMCSA CGLQAAVVCV TLLNRLEGDQ ISSPRNVLSE YQQRPQRLVS YFIKKKLSKA Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
