Influenza B Virus Antibody (FluB) (IgA) BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-351654
Hersteller Artikelnummer:
351654
Alternativnummer:
USB-351654-48,USB-351654-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
Sample Type: Serum Intended Use: For the qualitative determination of human influenza B virus (FluB) antibody (IgA) concentrations in serum. Specificity: This assay has high sensitivity and excellent specificity for detection of human influenza B virus (FluB) antibody(IgA). No significant cross-reactivity or interference between human influenza B virus (FluB) antibody(IgA) and analogues was observed. Test Principle: This BioAssay(TM) employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgA conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human influenza B virus (FluB) antibody(IgA) bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Coated assay plate, 1x96 wells Negative Control, 1x1ml Positive Control, 1x1ml HRP-conjugate, 1x10ml Sample Diluent, 1x20ml Wash Buffer (25X), 1x20ml Substrate A, 1x5ml Substrate B, 1x5ml Stop Solution, 1x5ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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