Tissue Inhibitors of Metalloproteinase 4 (TIMP-4) BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-351709
Hersteller Artikelnummer:
351709
Alternativnummer:
USB-351709-48,USB-351709-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling. Sample Type: Serum, plasma, tissue homogenates, human milk Intended Use: For the quantitative determination of human tissue inhibitors of metalloproteinase 4 (TIMP-4) concentrations in serum, plasma, tissue homogenates. Sensitivity: 0.005ng/ml Range: 0.313-20ng/ml Specificity: This assay has high sensitivity and excellent specificity for detection of human TIMP-4. No significant cross-reactivity or interference between human TIMP-4 and analogues was observed. Test Principle: This BioAssay(TM) employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TIMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TIMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TIMP-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TIMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured. Kit Components: Assay plate (12x8 coated microwells), 1x96 wells Standard (Freeze dried), 2x vials Biotin-antibody (100X), 1x120ul HRP-avidin (100X), 1x120ul Biotin-antibody Diluent, 1x15ml HRP-avidin Diluent, 1x15ml Sample Diluent, 1x50ml Wash Buffer (25X), 1x20ml TMB Substrate, 1x10ml Stop Solution, 1x10ml Adhesive Strip (For 96 wells), 4x strips Instruction manual Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
