ADAMTS1, BioAssay(TM) ELISA Kit (Human) (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif 1)
Artikelnummer:
USB-352694
Hersteller Artikelnummer:
352694
Alternativnummer:
USB-352694-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
ADAMTS1 (A Disintegrin-Like and Metalloproteinase with Thrombospondin Type 1 Motif, 1), also known as METH1, is an enzyme that in humans is encoded by the ADAMTS1 gene. ADAMTS is a family of proteins believed to be anchored to the extracellular matrix (ECM) through interactions with aggregan or other matrix components by one or more thrombospondin type 1 motifs. ADAMTS1 gene is mapped to 21q21.2 based on sequence similarity between the ADAMTS1 sequence and a chromosome 21q21.2 clone. It has been found that ADAMTS1 can disrupt angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. ADAMTS1 disrupts angiogenesis in vivo and in vitro more efficiently than ADAMTS8, THBS1, or endostatin. Sample Type: Cell culture supernates, serum and plasma (heparin) Intended Use: For quantitative detection of human ADAMTS1 in cell culture supernates, serum and plasma (heparin). Sensitivity: <10pg/ml Range: 93.8-6000pg/ml Specificity: Natural and recombinant human ADAMTS1 Test Principle: This human ADAMTS1 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for ADAMTS1 has been precoated onto 96-well plates. Standards(NSO, F253-A734) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for ADAMTS1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human ADAMTS1 amount of sample captured in plate. Kit Components: 96-well plate precoated with human ADAMTS1 antibody, 1 Lyophilized recombinant human ADAMTS1 standard, 10ng/tube*2 Biotinylated human ADAMTS1 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
