CD6 (Cluster of Differentiation 6), BioAssay(TM) ELISA Kit (Mouse)
Artikelnummer:
USB-352773
Hersteller Artikelnummer:
352773
Alternativnummer:
USB-352773-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
CD6 (Cluster of Differentiation 6) is a human protein encoded by the CD6 gene. It is mapped to 11q12.2. CD6 is expressed predominantly in peripheral T cells and mature medullary thymocytes. This gene encodes a protein found on the outer membrane of T-lymphocytes as well as some other immune cells. The encoded protein contains three scavenger receptor cysteine-rich (SRCR) domains and a binding site for an activated leukocyte cell adhesion molecule. The gene product is important for continuation of T cell activation. This gene may be associated with susceptibility to multiple sclerosis. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA) Intended Use: For quantitative detection of mouse CD6 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <10pg/ml Range: 62.5-4000pg/ml Specificity: Natural and recombinant mouse CD6 Test Principle: This mouse CD6 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD6 has been precoated onto 96-well plates. Standards(NSO, L18-G396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD6 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD6 amount of sample captured in plate. Kit Components: 96-well plate precoated with mouse CD6 antibody, 1 Lyophilized recombinant mouse CD6 standard, 10ng/tube*2 Biotinylated mouse CD6 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
