Chemokine (C-X-C motif) ligand 7 (CXCL7), also called PPBP or SCYB7, is a human gene. The encoded protein, Chemokine(C-X-C motif) ligand is a small cytokine belonging to the CXC chemokine family. CXCL7 is mapped to 4q13.3. It is a protein that is released in large amounts from platelets following their activation. CXCL7 is the precursor of the 2 platelet alpha-granule proteins, platelet basic protein(PBP) and connective tissue-activating peptide III(CTAP3). It stimulates various processes including mitogenesis, synthesis of extracellular matrix, glucose metabolism and synthesis of plasminogen activator. It also stimulates the formation and secretion of plasminogen activator by synovial cells. Sample Type: Cell culture supernates, serum and plasma (heparin, EDTA) Intended Use: For quantitative detection of human CXCL7 in cell culture supernates, serum and plasma (heparin, EDTA). Sensitivity: <2pg/ml Range: 15.6-1000pg/ml Specificity: Natural and recombinant human CXCL7 Test Principle: This human CXCL7 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL7 has been precoated onto 96-well plates. Standards(E.coli) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL7 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL7 amount of sample captured in plate. Kit Components: 96-well plate precoated with human CXCL7 antibody, 1 Lyophilized recombinant human CXCL7 standard, 10ng/tube*2 Biotinylated human CXCL7 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
