Cysteine Rich Secretory Protein 3 (CRISP3), BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-352815
Hersteller Artikelnummer:
352815
Alternativnummer:
USB-352815-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
Cysteine-rich secretory protein 3 is a cysteine-rich secretory protein that in humans is encoded by the CRISP3 gene. CRISP3 expression is found in the secretory epithelium throughout the male genital tract, with particularly high expression in the cauda epididymis and ampulla vas deferens. It is a potential biomarker for prostate cancer. CRISP3 gene can be defined as an early-transcribed gene with a putative role in the pathophysiology of sjogrens syndrome. CRISP3 is present in exocrine secretions and in secretory granules of neutrophilic granulocytes and is believed to play a role in innate immunity. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates Intended Use: For quantitative detection of human CRISP3 in cell culture supernates, serum, plasma (heparin, EDTA), saliva, urine and tissue homogenates. Sensitivity: <50pg/ml Range: 1.56-100ng/ml Specificity: Natural and recombinant human CRISP3 Test Principle: This human CRISP3 BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CRISP3 has been precoated onto 96-well plates. Standards(NSO, N21-Y245) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CRISP3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CRISP3 amount of sample captured in plate. Kit Components: 96-well plate precoated with human CRISP3 antibody, 1 ST0000-100, Lyophilized recombinant human CRISP3 standard, 100ng/tube*2 Biotinylated human CRISP3 antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
