Interleukin 18 Binding Protein (IL18BP), BioAssay(TM) ELISA Kit (Human)
Artikelnummer:
USB-352946
Hersteller Artikelnummer:
352946
Alternativnummer:
USB-352946-96
Hersteller:
US Biological
Kategorie:
Kits/Assays
Applikation:
ELISA
Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and urine Intended Use: For quantitative detection of human IL-18BP in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Sensitivity: <10pg/ml Range: 93.7-6000pg/ml Specificity: Natural and recombinant human IL-18BP Test Principle: This human IL-18BP BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate. Kit Components: 96-well plate precoated with human IL-18BP antibody, 1 Lyophilized recombinant human IL-18BP standard, 10ng/tube*2 Biotinylated human IL-18BP antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
