BIRC7, also called KIAP or Livin, is a protein that in humans is encoded by the BIRC7 gene. This gene is mapped to 20q13.33. It encodes a member of the inhibitor of apoptosis protein (IAP) family, and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING-type zinc finger domain. The BIR domain is essential for inhibitory activity and interacts with caspases, while the RING finger domain sometimes enhances antiapoptotic activity but does not inhibit apoptosis alone. Elevated levels of the encoded protein may be associated with cancer progression and play a role in chemotherapy sensitivity. BIRC7 blocked apoptosis induced by BAX induction of cytochrome C release from mitochondria, and it also can block apoptosis induced by chemotherapeutic agents. Sample Type: Cell culture supernates, serum, cell lysates and tissue homogenates Intended Use: For quantitative detection of human Livin in cell culture supernates, serum, cell lysates and tissue homogenates. Sensitivity: <20pg/ml Range: 0.78-50ng/ml Specificity: Natural and recombinant human Livin Test Principle: This human Livin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Livin has been precoated onto 96-well plates. Standards(E.coli, M1-S298) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Livin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Livin amount of sample captured in plate. Kit Components: 96-well plate precoated with human Livin antibody, 1 ST0000-100, Lyophilized recombinant human Livin standard, 100ng/tube*2 Biotinylated human Livin antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
