| Renin, also known as an angiotensinogenase, is an enzyme that participates in the bodys renin-angiotensin system(RAS). This gene is mapped to 1q32.1. Renins primary function is to cause an increase in blood pressure, leading to restoration of perfusion pressure in the kidneys. It also can catalyze the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Renin secretion is also stimulated by sympathetic nervous stimulation, mainly through beta-1 adrenoceptor activation. Sample Type: Cell culture supernates, serum, plasma (heparin, EDTA) and urine Intended Use: For quantitative detection of human Renin in cell culture supernates, serum, plasma (heparin, EDTA) and urine. Sensitivity: <10pg/ml Range: 31.2-2000pg/ml Specificity: Natural and recombinant human Renin Test Principle: This human Renin BioAssay(TM) ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Renin has been precoated onto 96-well plates. Standards(NSO, L67-R406) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Renin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Renin amount of sample captured in plate. Kit Components: 96-well plate precoated with human Renin antibody, 1 Lyophilized recombinant human Renin standard, 10ng/tube*2 Biotinylated human Renin antibody, 130ul (dilution 1:100) Avidin-Biotin-Peroxidase Complex (ABC), 130ul (dilution 1:100) Sample diluent buffer, 30ml Antibody diluent buffer, 12ml ABC diluent buffer, 12ml TMB color developing agent, 10ml TMB stop solution, 10ml Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
