| S-adenosylhomocysteine ELISA kit. S-Adenosyl-L-homocysteine (SAH) is an aa derivative and an intermediate, by-product, or modulator of several metabolic pathways, including the activated methyl cycle and cysteine biosynthesis. It is also a product of S-adenosyl-methionine (SAM)-dependent methylation of biological molecules, including DNA, RNA, and histones and other proteins. SAH is a risk factor for many diseases, including cancer and neurodegenerative diseases. Sample Type: Yeast and other expression system, tissue Intended Use: This BioAssay(TM) kit is used to measure the level of SAH in samples of soluble form. Applications: Methylation capability, one-carbon metabolism, methylation index Sensitivity: 10-20nM Range: 31.25-1000nM Specificity: S-adenosylhomocysteine (SAH) Kit Components: Wash Solution, 20x, 1x20ml Assay Plate, 1x96 wells HRP-antibody, 1x15-17ul HRP-antibody Diluent, 1x7.5 Sample Diluent, 1x6ml TMB Substrate, 1x11ml Stop Solution, 1x6ml Microplate Sealers, 2 S0 SAH Standard, 0nM, S1 SAH Standard, 31.25nM, 1x330ul S2 SAH Standard, 62.5nM, 1x660ul S3 SAH Standard, 125nM, 1x330ul S4 SAH Standard, 250nM, 1x330ul S5 SAH Standard, 500nM, 1x330ul S6 SAH Standard, 1000nM, 1x330ul QC SAH Quality Control, 320-480nM, 1x330ul Storage and Stability: Store powder at 4C liquid at -20C. Store other components at 4C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |